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Mirus Bio.jpg

Mirus Bio公司是世界上最早开发高效、低细胞毒性转染试剂的公司之一,同时也是目前该类试剂最主要的供应商之一。Mirus Bio的大部分转染试剂可以在含有血清的培养基中进行转染,省去了更换培养基的麻烦。TransIT-L1是Mirus产品的王牌,自1995年开发出来就申请了美国专利(US 5,744,335)。该项专利技术后来License给了Roche公司,以Fugene 6的品牌对外销售。TransIT-TKO & siQUEST转染试剂是常规siRNA转染试剂的王牌,与脂质体介导的转染试剂相比,该转染试剂可以高效转染siRNA,并且极大地降低了细胞的损伤程度。该试剂与siRNA混合转染细胞时,可以特异性剔除某些基因的表达,用于siRNA介导的基因沉默现象的研究。TransIT-Oligo转染试剂是即用型试剂,可以高效地将各种类型的寡聚核苷酸和寡聚脱氧核苷酸转染至受体细胞中。

 

此外,Mirus Bio还开发了专门针对特殊细胞的体外转染试剂,例如分别针对Jurkat,COS,3T3等细胞的转染试剂。Mirus Bio的科学家们新近开发了一种新的DNA转染试剂:TransITTM-2020 Transfection Reagent。TransITTM的性能优于FuGENETM、LipofectamineTM 2000和 LipofectamineTM 2000 CD;可在多种类型的细胞(包括难转染细胞)中实现高表达。

 

Mirus was founded on the principle that non-viral (i.e. plasmid DNA based) methods offer distinct advantages over viral methods of gene transfer. Our initial breakthrough in 1996 came with the development of a new type of transfection reagent that combined high efficiency delivery with low cellular toxicity: TransIT®-LT1 (Low Toxicity) Reagent remains the “gold standard” for broad-based gene delivery applications.

 

Mirus Bio公司主要产品:

Transfection with TransIT

Ingenio® Electroporation

Virus Production

Labeling with Label IT®

In Vivo Delivery

Accessory Products


品名货号
Human IgG1 Expression Control – 100 ugMIR 6250
Ingenio EZporator Electroporation SystemMIR 51000
Label IT Nucleic Acid Labeling Kit, Amine – sufficient for modifying 25 µg of nucleic acidMIR 3925
Label IT Nucleic Acid Labeling Kit, Amine – sufficient for modifying 100 µg of nucleic acidMIR 3900
TransIT-2020 Transfection Reagent – 0.4 mLMIR 5404
TransIT-2020 Transfection Reagent – 1.0 mLMIR 5400
TransIT-2020 Transfection Reagent – 10 X 1.0 mLMIR 5406
TransIT-2020 Transfection Reagent – 5 X 1.0 mLMIR 5405
TransIT-293  Transfection Reagent – 0.4 mLMIR 2704
TransIT-293 Transfection Reagent – 1.0 mLMIR 2700
TransIT-293 Transfection Reagent – 10 X 1.0 mLMIR 2706
TransIT-293 Transfection Reagent – 5 X 1.0 mLMIR 2705
TransIT-CHO  Transfection Kit – 10 X 1.0 mLMIR 2176
TransIT-CHO  Transfection Kit – 5 X 1.0 mLMIR 2175
TransIT-CHO Transfection Kit – 1.0 mLMIR 2170
TransIT-HeLaMONSTER Transfection Kit – 0.4 mLMIR 2904
TransIT-HeLaMONSTER Transfection Kit – 1.0 mLMIR 2900
TransIT-HeLaMONSTER Transfection Kit – 10 X 1.0 mLMIR 2906
TransIT-HeLaMONSTER Transfection Kit – 5 X 1.0 mLMIR 2905
TransIT-Insect Transfection Reagent – 0.4 mLMIR 6104
TransIT-Insect Transfection Reagent – 1.0 mLMIR 6100
TransIT-Insect Transfection Reagent – 10 X 1.0 mLMIR 6106
TransIT-Insect Transfection Reagent – 5 X 1.0 mLMIR 6105
TransIT-Jurkat Transfection Reagent – 0.4 mLMIR 2124
TransIT-Jurkat Transfection Reagent – 1.0 mLMIR 2120
TransIT-Jurkat Transfection Reagent – 10 X 1.0 mLMIR 2126
TransIT-Jurkat Transfection Reagent – 5 X 1.0 mLMIR 2125
TransIT-Keratinocyte Transfection Reagent – 0.4 mLMIR 2804
TransIT-Keratinocyte Transfection Reagent – 1.0 mLMIR 2800
TransIT-Keratinocyte Transfection Reagent – 10 X 1.0 mLMIR 2806
TransIT-Keratinocyte Transfection Reagent – 5 X 1.0 mLMIR 2805
TransIT-LT1 Transfection Reagent – 0.4 mLMIR 2304
TransIT-LT1 Transfection Reagent – 5 X 1.0 mLMIR 2305
TransIT-mRNA Transfection Kit – 0.4 mLMIR 2225
TransIT-mRNA Transfection Kit – 1.0 mLMIR 2250
TransIT-mRNA Transfection Kit – 10 X 1.0 mLMIR 2256
TransIT-mRNA Transfection Kit – 5 X 1.0 mLMIR 2255
TransIT-Oligo Transfection Reagent – 0.4 mLMIR 2164
TransIT-Oligo Transfection Reagent – 1.0 mLMIR 2160
TransIT-Oligo Transfection Reagent – 10 X 1.0 mLMIR 2166
TransIT-Oligo Transfection Reagent – 5 X 1.0 mLMIR 2165
TransIT-PRO Transfection Kit – 1.0 mL (with boost)MIR 5700
TransIT-PRO Transfection Kit – 10 mL (with boost)MIR 5760
TransIT-PRO Transfection Reagent – 1 mL (without boost)MIR 5740
TransIT-PRO Transfection Reagent – 10 mL (without boost)MIR 5750
TransIT-siQUEST Transfection Reagent – 0.4 mLMIR 2114
TransIT-siQUEST Transfection Reagent – 1.5 mLMIR 2110
TransIT-siQUEST Transfection Reagent – 10 X 1.5 mLMIR 2116
TransIT-siQUEST Transfection Reagent – 5 X 1.5 mLMIR 2115
TransIT-TKO Transfection Reagent – 0.4 mLMIR 2154
TransIT-TKO Transfection Reagent – 1.5 mLMIR 2150
TransIT-TKO Transfection Reagent – 10 X 1.5 mLMIR 2156
TransIT-TKO Transfection Reagent – 5 X 1.5 mLMIR 2155
TransIT-X2 Dynamic Delivery System – 0.3 mLMIR 6003
TransIT-X2 Dynamic Delivery System – 0.75 mLMIR 6004
TransIT-X2 Dynamic Delivery System – 1.5mLMIR 6000
TransIT-X2 Dynamic Delivery System – 10 X 1.5 mLMIR 6006
TransIT-X2 Dynamic Delivery System – 5 X 1.5 mLMIR 6005
Ingenio Electroporation Accessory – Cell Droppers (50 pk)MIR 50125
Ingenio Electroporation Solution Only – 25 rxns (6.25 mL)MIR 50111
Label IT Nucleic Acid Labeling Kit, Biotin – labels 100 µg nucleic acidMIR 3400
Label IT Plasmid Delivery Control, Cy3 – 10 µgMIR 7904
Label IT RNAi Delivery Control, Cy3 – 10 µg (0.75 nmol)MIR 7900
TransduceIT Transduction Reagent – 1 mLMIR 6620
TransIT-BrCa Reagent Transfection Reagent – 0.4 mLMIR 5504
Ingenio Electroporation Accessory – 0.4 cm Cuvettes (50 pk)MIR 50123
Ingenio Electroporation Solution Only – 50 rxns (12.5 mL)MIR 50114
Label IT Nucleic Acid Labeling Kit, Biotin – labels 25 µg nucleic acidMIR 3425
Label IT Plasmid Delivery Control, Cy3 – 100 µgMIR 7905
Label IT RNAi Delivery Control, Cy3 – 100 µg (7.5 nmol)MIR 7901
Label IT siRNA Tracker Intracellular Localization Kit, CX-Rhodamine – labels 50 µg siRNAMIR 7214
Label IT Tracker Intracellular Nucleic Acid Localization Kit, CX-Rhodamine – labels 50-200 µg plasmid DNAMIR 7022
Lentivirus Packaging Mix – 34 rxnMIR 6640
TransIT-BrCa Reagent Transfection Reagent – 1.0 mLMIR 5500
TransIT-LT1 Transfection Reagent – 1.0 mLMIR 2300
Ingenio Electroporation Accessory – 0.2 cm Cuvettes (50 pk)MIR 50121
Ingenio Electroporation Solution Only – 100 rxns (25 mL)MIR 50117
Label IT Nucleic Acid Labeling Kit, CX-Rhodamine – labels 100 µg nucleic acidMIR 3100
Label IT Plasmid Delivery Control, Fluorescein – 10 µgMIR 7906
Label IT RNAi Delivery Control, Fluorescein – 10 µg (0.75 nmol)MIR 7902
Label IT siRNA Tracker Intracellular Localization Kit, Cy3 – labels 50 µg siRNAMIR 7212
Label IT Tracker Intracellular Nucleic Acid Localization Kit, Cy3 – labels 50-200 µg plasmid DNAMIR 7020
Lentivirus Packaging Mix – 5 rxnMIR 6630
TransIT-BrCa Transfection Reagent – 5 x 1.0 mLMIR 5505
Ingenio Electroporation Kit, for Amaxa – 25 rxns (0.2 cm Cuvettes/Droppers)MIR 50112
Label IT Nucleic Acid Labeling Kit, CX-Rhodamine – labels 25 µg nucleic acidMIR 3125
Label IT Plasmid Delivery Control, Fluorescein – 100 µgMIR 7907
Label IT RNAi Delivery Control, Fluorescein – 100 µg (7.5 nmol)MIR 7903
Label IT siRNA Tracker Intracellular Localization Kit, Cy5 – labels 50 µg siRNAMIR 7213
Label IT Tracker Intracellular Nucleic Acid Localization Kit, Cy5 – labels 50-200 µg plasmid DNAMIR 7021
TransIT Lentivirus System – 34 rxnMIR 6655
TransIT-BrCa Transfection Reagent – 10 x 1.0 mLMIR 5506
TransIT-LT1 Transfection Reagent – 10 X 1.0 mLMIR 2306
Ingenio Electroporation Kit, for Amaxa – 50 rxns (0.2 cm Cuvettes/Droppers)MIR 50115
Label IT Nucleic Acid Labeling Kit, Cy3 – labels 100 µg nucleic acidMIR 3600
Label IT siRNA Tracker Intracellular Localization Kit, Fluorescein – labels 50 µg siRNAMIR 7216
Label IT Tracker Intracellular Nucleic Acid Localization Kit, Fluorescein – labels 50-200 µg plasmid DNAMIR 7025
TransIT Lentivirus System – 5 rxnMIR 6650
Ingenio Electroporation Kit, for Amaxa – 100 rxns (0.2 cm Cuvette/Droppers)MIR 50118
Label IT Nucleic Acid Labeling Kit, Cy3 – labels 25 µg nucleic acidMIR 3625
TransIT-Lenti Transfection Reagent – 10 x 1.5 mLMIR 6606
Ingenio Electroporation Kit, for other Electroporators – 25 rxns, (0.4 cm Cuvettes/Droppers)MIR 50113
Label IT Nucleic Acid Labeling Kit, Cy5 – labels 100 µg nucleic acidMIR 3700
TransIT-Lenti Transfection Reagent – 1.5 mLMIR 6600
Ingenio Electroporation Kit, for other Electroporators – 50 rxns (0.4 cm Cuvettes/Droppers)MIR 50116
Label IT Nucleic Acid Labeling Kit, Cy5 – labels 25 µg nucleic acidMIR 3725
TransIT-Lenti Transfection Reagent – 0.75 mLMIR 6604
Ingenio Electroporation Kit, for other Electroporators – 100 rxns (0.4 cm Cuvette/Droppers)MIR 50119
TransIT-Lenti Transfection Reagent – 5 x 1.5 mLMIR 6605
TransIT-Lenti Transfection Reagent – 0.3 mLMIR 6603
Label IT Nucleic Acid Labeling Kit, DNP – labels 100 µg nucleic acidMIR 3800
Label IT Nucleic Acid Labeling Kit, DNP – labels 25 µg nucleic acidMIR 3825
Label IT Nucleic Acid Labeling Kit, Fluorescein – labels 100 µg nucleic acidMIR 3200
Label IT Nucleic Acid Labeling Kit, Fluorescein – labels 25 µg nucleic acidMIR 3225
Label IT Nucleic Acid Labeling Kit, MFP488 – labels 100 µg nucleic acidMIR 7100
Label IT Nucleic Acid Labeling Kit, MFP488 – labels 25 µg nucleic acidMIR 7125
TransIT-EE Delivery Solution – 40 InjectionsMIR 5340
TransIT-QR Delivery Solution – 40 InjectionsMIR 5240
pLIVE Vector – 20 ugMIR 5420
pLIVE Vector/LacZ – 20 ug of each vectorMIR 5520
pLIVE Vector/SEAP Control Vector Kit – 20 ug of each vectorMIR 5620
pLIVE Vector Complete System – 20 ug of each vectorMIR 5320
RevIT AAV Enhancer – 1.5 mL (for 1 L of cell culture)MIR 8000
TransIT-VirusGEN GMP Transfection Reagent – 150 mLMIR 6845-GMP
VirusGEN AAV Transfection Kit – 150mLMIR 6755
VirusGEN AAV Transfection Kit, 1 Kit – for 10 L of cell cultureMIR 6745
RevIT AAV Enhancer – 10 x 1.5 mL (for 10 L of cell culture)MIR 8006
RevIT AAV Enhancer – 75 mL (for 50 L of cell culture)MIR 8080
VirusGEN GMP LV Transfection KitMIR 6825-GMP
VirusGEN LV Transfection KitMIR 6760
VirusGEN AAV Kit + RevIT for 10L CultureMIR 8008
VirusGEN GMP AAV Transfection KitMIR 6815-GMP
VirusGEN AAV Transfection Kit – 1 Kit for 1 L of cell cultureMIR 6750
VirusGEN AAV Kit + RevIT for 1L CultureMIR 8007
TransIT-VirusGEN Transfection Reagent – 150 mLMIR 6740
TransIT-VirusGEN Transfection Reagent – 30 mLMIR 6720
TransIT-VirusGEN Transfection Reagent – 10 x 1.5 mLMIR 6706
TransIT-VirusGEN Transfection Reagent – 5 x 1.5 mLMIR 6705


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全国服务热线:400-6800-868

邮箱:sales@amyjet.com

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Logo-R-Website.pngexocell.jpg

艾美捷科技代理Ligatrap品牌全系列产品


LigaTrap Technologies是一家位于北卡罗来纳州的创新型生物技术公司,提供独特的专利技术组合,专注于蛋白质纯化领域。LigaTrap精于抗体与病毒纯化,服务于抗体药物、病毒疫苗及基因治疗产业。


▍LigaTrap公司产品

● LigaGuard:最新推出的亲和吸附剂,是由多模态配体构成的专利复合体系,可在流穿模式(连续流)下选择性捕获宿主细胞蛋白(HCPs)与DNA。

● AAVidity™:一种新型亲和层析填料,用于血清型普适性纯化 源自 HEK293 和 Sf9 细胞裂解液 的 腺相关病毒(AAV)。

● IgM Resin:专为纯化 IgM 抗体设计的亲和树脂,具有高纯度、高结合能力、温和的洗脱条件(pH 约 4.0),并可重复使用多达 100 次。

● Base Resin:专为从多克隆与单克隆来源中纯化高质量抗体而设计,该树脂推荐用于纯化:多种物种的 IgG(包括人、小鼠、兔等);人源 IgA;鸡源 IgY。

● LigaTrap Buffers:包含Regeneration Buffer,Neutralization Buffer,Elution Buffer,Regeneration Buffer,Equilibration/Wash Buffer 2.0等。


Ligatrap.png


▍LigaGuard

LigaGuard™树脂适用于两类核心产品的纯化:蛋白制品:单克隆抗体、生长因子;病毒制品:慢病毒、腺相关病毒(AAV)、病毒疫苗。该树脂的设计使目标生物产物无结合通过,实现高纯度与高回收率。其能清除传统纯化工艺难以去除的高残留性HCPs(HR-HCPs),显著提升终产物的纯度、稳定性、疗效与安全性。

品名货号规格
LigaTrap LigaGuard 1 ml Packed ColumnLT-LG1-1x1mL1mL
LigaTrap LigaGuard 5ml Packed ColumnLT-LG1-1x5mL5mL
LigaTrap LigaGuard Loose Resin - 5mLLT-LG15 mL
LigaTrap LigaGuard Loose Resin - 10mLLT-LG1-10mL10 mL
LigaTrap LigaGuard Loose Resin - 25mLLT-LG1-25mL25 mL
LigaTrap LigaGuard Loose Resin - 50mLLT-LG1-50mL50 mL
LigaTrap LigaGuard Loose Resin - 100mLLT-LG1-100mL100 mL


▍AAVidity

AAVidity填料通过合成亲和配体功能化修饰,可捕获所有血清型的 AAV,提供卓越的结合载量与污染物(宿主细胞蛋白 HCPs 及宿主细胞DNA hcDNA清除能力。其温和洗脱条件在保障 AAV 转导活性的同时,实现高产物回收率。

品名货号规格
LigaTrap AAVidity Loose Resin – 1mLLT-AAV-1mL1mL
LigaTrap AAVidity Loose Resin – 5mLLT-AAV-5mL5mL
LigaTrap AAVidity Prepacked Column – 1mLLT-AAV-1x1mL1x1mL
LigaTrap AAVidity Prepacked Column – 5mLLT-AAV-1x5mL1x5mL


▍IgM Resin

 IgM纯化树脂的核心优势包括:

● 全面捕获:同时结合IgM的重链与轻链亚型;

● 零风险:无免疫原性及配体脱落;

● 活性保全:温和洗脱条件保障抗体完整性;

● 经济耐用:消毒再生后可重复使用 ≥100 次;

● 该树脂能高效处理并纯化来自以下样本的单克隆 IgM:细胞培养上清,腹水,杂交瘤,其他重组 IgM 来源。

品名货号规格
IgM Microspin Column Kit w/ BuffersLT-155KITKIT-10x0.1mL
IgM 1mL Prepacked Column Kit w/ BuffersLT-155-1mL KITKIT-1x1mL
IgM 5mL Prepacked Column Kit w/ BuffersLT-155-5mL KITKIT-1x5mL
IgM Loose Resin – 5mLLT-1555mL
IgM Loose Resin – 10mLLT-155-10mL10mL
IgM Loose Resin – 25mLLT-155-25mL25mL
IgM Loose Resin – 50mLLT-155-50mL50mL
IgM Loose Resin – 100mLLT-155-100mL100mL
IgM Microspin Columns without BuffersLT-155-MSC10x0.1mL
IgM Prepacked Column – 1mLLT-155-1x1mL1x1mL
IgM Prepacked Column – 5mLLT-155-1x5mL1x5mL
IgM Prepacked Columns – 3x1mLLT-155-3x1mL3x1mL

▍Base Resin

LigaTrap基础纯化树脂可实现高回收率与>95%的纯度,而传统Protein A和Protein G填料的回收率甚至低于检测限。通过优化工艺,该树脂的纯度可进一步提升至99%。

品名货号规格
Base Resin Microspin Column Kit w/ BuffersLT-150KITKIT-10x0.1mL
Base Resin 1mL Prepacked Column Kit w/ BuffersLT-150-1mL KITKIT-1x1mL
Base Resin 5mL Prepacked Column Kit w/ BuffersLT-150-5mL KITKIT-1x5mL
Loose Base Resin – 5mLLT-1505mL
Loose Base Resin – 10mLLT-150-10mL10mL
Loose Base Resin – 25mLLT-150-25mL25mL
Loose Base Resin – 50mLLT-150-50mL50mL
Loose Base Resin – 100mLLT-150-100mL100mL
Base Resin Microspin Columns without BuffersLT-150-MSC10x0.1mL
Base Resin Prepacked Column – 1mLLT-150-1x1mL1x1mL
Base Resin Prepacked Column – 5mLLT-150-1x5mL1x5mL
Base Resin Prepacked Columns – 3x1mLLT-150-3x1mL3x1mL


▍Buffers

品名货号规格
LigaTrap Elution BufferBU-123-FP125ml
LigaTrap Regeneration BufferBU-124-FP50ml
LigaTrap Neutralization BufferBU-125-FP15ml
LigaTrap Storage BufferBU-126-FP50 ml
LigaTrap Sample Diluent 2.0BU-131-FP15 ml
LigaTrap Equilibration/Wash Buffer 2.0BU-132-FP250ml
LigaTrap IgM Elution Buffer pH 3.8BU-133-FP125 ml
LigaTrap Elution BufferBU-123-500-FP500ml
LigaTrap Regeneration BufferBU-124-500-FP500 ml
LigaTrap Neutralization BufferBU-125-250-FP250 ml
LigaTrap Storage BufferBU-126-500-FP500 ml
LigaTrap Sample Diluent 2.0BU-131-250-FP250 ml
LigaTrap Equilibration/Wash Buffer 2.0BU-132-1-FP1L
LigaTrap IgM Elution Buffer pH 3.8BU-133-500-FP500ml


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联系我们

地址:武汉市洪山区光谷大道35号光谷总部国际时代 二期1栋1301

电话:13212737804

传真:18771149750

邮箱:sales@amyjet.com

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